Fig. 7
Profile of infiltrating myeloid cells and their immunosuppression. Cell numbers of a macrophages, b granulocytic MDSCs (gMDSCs) and c monocytic (mMDSCs) in tumor tissues and spleens of tumor-bearing mice (TB) as well as in liver tissues and spleens of control normal C57BL/6j mice (ctrl) were shown. Proliferation of HBc93–100-specific CD8+ T cells (8 × 104/well) co-cultured with different numbers of d FACSorted tumor-associated macrophages (TAMs) and FACSorted splenic macrophages, respectively, or e FACSorted intra-tumoral myeloid-derived suppressor cells (MDSCs), and FACSorted splenic Gr-1+ myeloid cells, respectively. The proliferation of T cells was determined by EdU incorporation assay and the percentage of EdU+ CD8+ T cells among total CD8+ T cells was shown. ns, not significant; ***P < 0.001 (unpaired Student’s t-test)