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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: 1-Pyrroline-5-carboxylate released by prostate Cancer cell inhibit T cell proliferation and function by targeting SHP1/cytochrome c oxidoreductase/ROS Axis

Fig. 1

PCM Inhibit T Cell Proliferation, Function and T Cell Infiltration in PC and BPH Tissue. (a) CFSE-labeled human primary CD3+ T cells were pretreated with PCM or two normal cells media then stimulated for 3 days with anti-CD3/CD28 beads. T-cell proliferation was evaluated by FACS analysis. The right side of bar graph is the representative result of CD3+ T cells proliferation. (b) Jurkat cells were treated with PCM or two normal cells media for 24 h. Shown is the percentage of cell proliferation by CCK-8 assay. One representative experiment out of three performed. (c-i) Human primary CD3+ T cells were pretreated with PCM or two normal cells media then stimulated for 3 days with anti-CD3/CD28 beads. Supernatants from cell cultures were analyzed for seven cytokines levels using commercially available ELISA kits. One representative experiment out of three performed. (j) Columns showed the quantitative statistics of the infiltration of T cells. (k) The infiltration of T cells in PCa (n = 25) and BPH (n = 15) tissue detected by IF. The red light marked T cells. The representative pictures of IF. Error bars are SEM of biological replicates and ***p < 0.01

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