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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: 1-Pyrroline-5-carboxylate released by prostate Cancer cell inhibit T cell proliferation and function by targeting SHP1/cytochrome c oxidoreductase/ROS Axis

Fig. 3

The Effect of PCM on Expression and Translocation of SHP1 in T cells. (a-b) The mRNA expression of SHP1 in CD3+ T cells and Jurkat cells after treatment with PCM by qPCR. (c) Obtained T cells from human prostate tissue by laser capture microdissection, and checked SHP1 expression in T cells of BPH and PCa tissue by qPCR. (d-e) Western bolt showing the protein expression of SHP1 in CD3+ T cells and Jurkat cells after treatment with PCM. An antibody to β-actin was used as a loading control. (f) Western bolt showing the cytoplasm protein expression of SHP1 in Jurkat cells after treatment with PCM. An antibody to β-actin was used as a loading control. (g) Western bolt showing the nuclear protein expression of SHP1 in Jurkat cells after treatment with PCM. An antibody to Lamin B1 was used as a loading control. (h) Western bolt showing the mitochondria protein expression of SHP1 in Jurkat cells after treatment with PCM. An antibody to COX IV was used as a loading control. (i) The localization of SHP-1 in Jurkat cells after treatment with PCM by immunofluorescence. DAPI was used for marking nuclear. (j) The localization of SHP-1 in T cells of PCa and BPH tissue by confocal microscopy. DAPI was used for marking nuclear, and CD3 for marking T cells Error bars are SEM of biological replicates and *p < 0.05

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