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Fig. 7 | Journal for ImmunoTherapy of Cancer

Fig. 7

From: 1-Pyrroline-5-carboxylate released by prostate Cancer cell inhibit T cell proliferation and function by targeting SHP1/cytochrome c oxidoreductase/ROS Axis

Fig. 7

The Change of PRODH Expression Affect the Growth of Tumor and T cells Infiltration in vivo. (a) EL-4 cells were treated with RM-1 cultured media for 24 h. Shown is the percentage of cell proliferation by CCK-8 assay. One representative experiment out of three performed. (b) EL-4 cells were treated with RM-1 cultured media for 24 h. Shown is the levels of ROS by flow cytometry. (c) The expression of PRODH in RM-1 after transfection by qPCR. (d) The cell number of RM-1 after transfection by CCK-8 assay. (e) Mean of tumor volume measured at the indicated number of days after mice were treated. (f) Mean of body weight of mice measured at the indicated number of days after mice were treated. (g) The picture of tumors after harvesting. (h) The infiltration of CD3+ T cells in tumor tissue detected by IF. The red light marked T cells. (i) Columns showed the quantitative statistics of the infiltration of T cells. Error bars are SEM of biological replicates and *p < 0.05; **p < 0.01; ***p < 0.001

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