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Fig. 6 | Journal for ImmunoTherapy of Cancer

Fig. 6

From: A rare population of tumor antigen-specific CD4+CD8+ double-positive αβ T lymphocytes uniquely provide CD8-independent TCR genes for engineering therapeutic T cells

Fig. 6

Specificity of 19305DP TCR. a IFN-γ production from 19305DP-TCR or CD8SP-TCR-transduced CD8+ T cells against wild-type or alanine-substituted NY-ESO-1157-165 peptides at different concentration was investigated by intracellular cytokine staining. b Percentage of relative IFN-γ production on TCR-transduced CD8+ T cells against alanine-substituted peptides at peptide concentration of 0.1 μM. Data are represented as % recognition as compared to % IFN-γ-producing CD8+ T cells against wild-type NY-ESO-1157-165 peptide. c 19305DP-TCR-transduced T cells were cocultured with the indicated panel of normal human tissue-derived primary cells or melanoma cell lines for 24 h. Some primary cell lines were pulsed with NY-ESO-1157-165 peptide (+pep) before coculture. IFN-γ level in the supernatant was measured by ELISA

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