Fig. 6
From: EnanDIM - a novel family of L-nucleotide-protected TLR9 agonists for cancer immunotherapy
Persistent immunological anti-tumor memory induced by EnanDIM-C in two syngeneic tumor models. 10 mice per group were inoculated s.c. with either A20 lymphoma (a, b), or EMT-6 breast cancer (c, d) tumor cells. Established tumors (40 mm3, day 3 to day 7) were injected with EnanDIM-C or vehicle (i.tu.). Mean tumor growth + SEM (left), and Kaplan-Meier survival plots (right) are shown (log-rank analyses: A20, p < 0.0001; EMT-6, p < 0.0001). Mean tumor growth curves are continued until 50% of mice treated with vehicle have been sacrificed. Light blue bar on each x axis indicates the treatment period. b, surviving mice from the A20 tumor model as well as age-matched naïve mice were re-challenged with A20 cells (1st re-challenge) and surviving mice were subsequently re-challenged with CT26 cell (2nd re-challenge) as specified. d, surviving mice from the EMT-6 tumor model as well as age-matched naïve mice were re-challenged with EMT-6 cells (1st re-challenge) and surviving mice were subsequently re-challenged with CT26 cell (2nd re-challenge) as specified. Individual tumor growth of all mice is shown and respective tumor cell injections are marked. e, spleen cells from naïve mice and from mice surviving two EMT-6 and one CT26 tumor inoculation were collected, co-cultured with either EMT-6, CT26 cells, AH1 peptide or Renca cells and subjected to ELISpot assay to quantify the IFN-gamma secreting cells