Fig. 4
From: Tumor microenvironment modulation enhances immunologic benefit of chemoradiotherapy
CRT + CTX/L-NIL promotes intratumoral CD8+ T cell infiltration and activation. Established mEER tumors were treated with CRT and/or CTX/L-NIL and harvested after the first week of treatment, as shown in Fig. 1e, and tumor lymphocyte infiltrate was analyzed using quantitative multiplex immunofluorescence (qmIF; a-c). a Representative multiplex images of mEER tumors showing DAPI (nuclei, dark blue), EpCAM (tumor, red), CD8α (CD8+ T cells, cyan), and Granzyme B (T cell cytotoxic marker, green). b Pie-charts showing T cell subset fractions among total T cells for each treatment (fraction averaged across 5 images per tumor and n = 3/group). c Log2 fold change of Granzyme B (GzB)+ CD8+ cells normalized to control group (N = 1; n = 3/group; Tukey’s multiple comparison test). Bar graphs show mean +/− SD. *p < 0.05; **p < 0.01