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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Hyperspectral cell sociology reveals spatial tumor-immune cell interactions associated with lung cancer recurrence

Fig. 1

Hyperspectral imaging of multicolor immunohistochemistry. a Example of a lung tumor section that was stained with multicolor immunohistochemistry for CD3 (blue, black arrow), CD8 (dark brown, white arrow), CD79a (red) and counterstained with haematoxylin (nuclear stain) and imaged in white light. b Following hyperspectral imaging and spectral unmixing, nuclei were identified using the haematoxylin spectrum and are shown in blue. Nuclear boundaries were located using an edge location algorithm and are shown as red lines. c Voronoi diagrams were generated for each segmented cell and shown as red lines with dots as centers of cells. Cell neighbors are cell pairs that share a Voronoi edge. d The interaction between each neighbor is indicated by a black line connecting nuclei centres. In panels B-D, unstained cells are represented by a red centre, CD3+ T cells are represented by a blue centre, and CD3+ CD8+ T cells are represented by a green centre

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