Fig. 2

Quantification of cell sociology. Three examples where the neighbor fractions of a single cell per image is shown. Lines represent cell – cell interactions between neighbors to the central cell and the color of the lines represent the color of the neighbor cell. For cell sociology, all cells in the image would be analyzed in the same manner to determine average neighbor frequencies of each cell type. a An example of a red cell with five neighbors (cells that share a common Voronoi edge) is shown: one red neighbor (1/5), one blue neighbor (1/5), and three yellow neighbors (3/5). For the red cell in the centre, the neighbor frequency of red cells is 0.2, the neighbor frequency of blue cells is 0.2, and the neighbor frequency of yellow cells is 0.6. b An example of a red cell with eight neighbors. In this case, all neighbors are red generating a neighbor frequency of 1.0. c An example of a blue cell with 13 neighbors: 10 red neighbors, two blue neighbors, and one green neighbor. For the blue cell in the centre, the neighbor frequency of red cells is 0.769, the neighbor frequency of blue cells is 0.154, and the neighbor frequency of green cells is 0.077. Circles represent a fixed pixel distance from the assessed cell in each image