Fig. 4

Cell densities and cell sociology of recurrent and non-recurrent lung tumors. a Densities of CD3+ CD8- T cells (blue), CD3+ CD8+ T cells (green), and CD79a+ cells (pink) calculated from tumor epithelial areas were compared between recurrent (n = 11) and non-recurrent (n = 8) cases of lung adenocarcinoma. b Cell sociology of unstained (mostly tumor) cells with CD3+ CD8- T cell neighbors (blue), CD3+ CD8+ T cell neighbors (green), and CD79a+ cell neighbors (pink) was calculated across tumor epithelial areas. The mean neighbor frequency of these interactions was compared between recurrent and non-recurrent cases of lung adenocarcinoma. In all analyses a Mann-Whitney U test p < 0.05 was considered significant, and bars indicate mean ± standard deviation. c Cell sociology of non-recurrent (left) and recurrent (right) case of lung adenocarcinoma. Each plot shows example analyses of a single unstained cell (red) within 3 fields taken from one patient slide. Cell-neighbor interactions are represented by a line color coded to match the neighbor of the unstained cell of interest. These examples highlight differences in neighbor frequencies of individual tumor cells