Table 1 Salmonella strains and plasmids
From: The motility regulator flhDC drives intracellular accumulation and tumor colonization of Salmonella
Strain | Background | Genetic circuits | Description |
|---|---|---|---|
1. Sal | ΔmsbB, ΔpurI, Δxyl (VNP20009) | P lac /DsRed P SSEJ /GFP | Constitutive DsRed Intracellularly inducible GFP Additional file 1: Figure S1-A |
2. F-Sal | Sal | P lac /DsRed P SSEJ /GFP P BAD /flhDC | Arabinose Inducible flhDC Constitutive DsRed Intracellularly inducible GFP Additional file 1: Figure S1-B |
3. S-Sal | ΔsipB Sal | P lac /DsRed P SSEJ /GFP | Minimally Intracellularly Invasive Constitutive DsRed Intracellularly inducible GFP Additional file 1: Figure S1-A |
4. FS-Sal | ΔsipB Sal | P lac /DsRed P SSEJ /GFP P BAD /flhDC | Arabinose Inducible flhDC Minimally Intracellularly Invasive Constitutive DsRed Intracellularly inducible GFP Additional file 1: Figure S1-B |
5. Salmonella (control) | ΔmsbB, ΔpurI, Δxyl | P lac /GFP | Constitutive GFP Additional file 1: Figure S1-C |
6. Salmonella + pflhDC | ΔmsbB, ΔpurI, Δxyl | P lac /GFP P BAD /flhDC | Arabinose inducible flhDC Constitutive GFP Additional file 1: Figure S1-D |
7. ΔflgE | ΔmsbB, ΔpurI, Δxyl ΔflgE | P lac /GFP | Non-motile Constitutive GFP Additional file 1: Figure S1-C |
8. ΔflgE+pflhDC | ΔmsbB, ΔpurI, Δxyl ΔflgE | P lac /GFP P BAD /flhDC | Arabinose inducible flhDC Non-motile Constitutive GFP Additional file 1: Figure S1-D |