Table 1 Salmonella strains and plasmids
From: The motility regulator flhDC drives intracellular accumulation and tumor colonization of Salmonella
| Strain | Background | Genetic circuits | Description |
|---|---|---|---|
| 1. Sal |
ΔmsbB, ΔpurI, Δxyl (VNP20009) | P lac /DsRed P SSEJ /GFP |
Constitutive DsRed Intracellularly inducible GFP Additional file 1: Figure S1-A |
| 2. F-Sal | Sal | P lac /DsRed P SSEJ /GFP P BAD /flhDC |
Arabinose Inducible flhDC Constitutive DsRed Intracellularly inducible GFP Additional file 1: Figure S1-B |
| 3. S-Sal | ΔsipB Sal | P lac /DsRed P SSEJ /GFP |
Minimally Intracellularly Invasive Constitutive DsRed Intracellularly inducible GFP Additional file 1: Figure S1-A |
| 4. FS-Sal | ΔsipB Sal | P lac /DsRed P SSEJ /GFP P BAD /flhDC |
Arabinose Inducible flhDC Minimally Intracellularly Invasive Constitutive DsRed Intracellularly inducible GFP Additional file 1: Figure S1-B |
|
5. Salmonella
(control) | ΔmsbB, ΔpurI, Δxyl | P lac /GFP |
Constitutive GFP Additional file 1: Figure S1-C |
| 6. Salmonella + pflhDC | ΔmsbB, ΔpurI, Δxyl |
P
lac
/GFP
P BAD /flhDC |
Arabinose inducible flhDC Constitutive GFP Additional file 1: Figure S1-D |
| 7. ΔflgE | ΔmsbB, ΔpurI, Δxyl ΔflgE | P lac /GFP |
Non-motile Constitutive GFP Additional file 1: Figure S1-C |
| 8. ΔflgE+pflhDC | ΔmsbB, ΔpurI, Δxyl ΔflgE |
P
lac
/GFP
P BAD /flhDC |
Arabinose inducible flhDC Non-motile Constitutive GFP Additional file 1: Figure S1-D |