Skip to main content
Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Different role of circulating myeloid-derived suppressor cells in patients with multiple myeloma undergoing autologous stem cell transplantation

Fig. 3

The influence of pre- and post-ASCT MDSC phenotypes on in vitro melphalan-induced cytotoxic assay. MM cell line, IM-9 cells (a) or primary MM cells (b) were cultured with or without MDSCs isolated from pre- and post-ASCT samples (MM cell:MDSC ratio 1:1) in the presence of human M-CSF. The top figures are representative staining with Annexin V-APC and PI after incubation with or without melphalan. In the bottom figure, individual data from independent melphalan-induced cytotoxic assay by E- and M-MDSC phenotypes isolated from five patients were compared. The label of post-ASCT MDSCs on the figure means the cells expressing each MDSC phenotype. The data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001

Back to article page