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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Different role of circulating myeloid-derived suppressor cells in patients with multiple myeloma undergoing autologous stem cell transplantation

Fig. 5

The influence of CSF1R inhibition on melphalan-induced cytotoxicity attenuated by pre-ASCT M-MDSCs. The influence of BLZ945, a human CSF1R inhibitor, on cell apoptosis induced by melphalan was tested (a). IM-9 cells were cultured with or without MDSCs isolated from pre- and post-ASCT patients (MM:MDSC ratio 1:1) in the presence of human M-CSF, as shown in Fig. 3. The top figures are representative staining with Annexin V-APC and PI after incubation with vehicle, 10 uM melphalan with or without 500 nM BLZ945. In the bottom figure, individual data from independent experiments by E- and M-MDSC phenotypes isolated from five patients were compared. (b) Cytokines (IL-6, IGF1, VEGF, and M-CSF) in culture supernatants with pre- and post-ASCT MDSC phenotypes were measured, and the effects of BLZ945 treatment were compared. The concentrations of IL-6, IGF1, and VEGF in culture supernatants with pre-ASCT MDSCs (top) and post-ASCT MDSC phenotypes (bottom) are shown. (c) M-CSF concentrations in culture supernatants with pre-ASCT MDSCs (left) and post-ASCT MDSC phenotypes (right) are shown. MDSC phenotypes were isolated from six patients. The label of post-ASCT MDSCs on the figure means the cells expressing each MDSC phenotype. The data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001

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