Fig. 2

PDL1 Chimeric tumors are resistant to oncolytic therapy. a-d 4 × 10⁵ total cells comprising pure cultures of either B16/F10^scramble (WT: n = 10), or B16/F10^PDL1−/− (KO: n = 15), or either a 1:9 mix (10% n = 14), or a 1:1 mix (50% n = 14) of WT to KO cells were injected SQ into the left flank of syngeneic C57/B6 mice. 7, 9, and 11 days post tumor implantation; tumors were treated with IT injection of 1 × 10⁷ foci forming units of MYXV. Tumors were then monitored every other day for tumor growth and animals euthanized when tumors reached 15 mm in any direction. a Response of individual tumors to treatment. Data is displayed as percent tumor area (LxW) compared to tumor area immediately prior to initiation of treatment. b Tumor area (LxW) in individual mice at day 20 post tumor cell injection. Statistical significance was determined using unpaired students T-Test (* < 0.05, *** < 0.01). c Overall survival animal survival. Data represents summation of two individual experiments