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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Chimeric tumor modeling reveals role of partial PDL1 expression in resistance to virally induced immunotherapy

Fig. 3

Phenotype of PDL1 Chimeric tumors. a-c 4 × 105 total cells comprising pure cultures of either B16/F10scramble (WT: n = 7) or B16/F10PDL1−/− (KO: n = 9) or a 1:9 mix of WT to KO cells (Mix: n = 10) were injected SQ into the left flank of syngeneic C57/B6 mice. Tumors were then monitored every other day for tumor growth and animals euthanized when tumors reached 15 mm in any direction. a Tumor area (LxW) of individual tumors over time. b Tumor area (LxW) in individual mice at day 20 post tumor cell injection. Statistical significance was determined using unpaired students T-Test (*** < 0.01). c Overall animal survival. Data represents summation of two individual experiments. d C57/B6 mice were injected with WT (n = 15), KO (n = 17), or a 1:9 mix (n = 21) of cells as above. On day eight post injection, tumor cells were harvested, disassociated into single cells, and cellular composition analyzed using flow cytometry. Data represents summation of three independent experiments. Statistical significance was determined using unpaired students T-Test (* < 0.05,*** < 0.01)

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