Fig. 2

PD-1⁺ immune infiltrates have at least two subsets in relation to CD8 cells in TNBCs; the CD8⁺PD-1⁺ double positive subset and the CD8⁻PD-1⁺ subset. mIF labeled sections from two representative TNBC tissue samples show the PD-1⁺, CD8⁺, the CD8⁺PD-1⁺ double positive subset and the CD8⁻PD-1⁺ subset within the tumor microenvironment. mIF labeling for PD-1 (green), CD8 (red), Pan-cytokeratin (cyan) and DAPI (blue). a TNBC harbored high CD8⁺PD-1⁺ double positive subset. b TNBC harbored low CD8⁺PD-1⁺ double positive subset. c and d Higher magnification of the same region from images (a and b, respectively) shows CD8⁺PD-1⁺ double positive subset through double labeling of CD8 (red) and PD-1(green), indicated by white arrows. High levels of CD8⁺PD-1⁺ infiltrates, but not high levels of CD8⁻PD-1⁺ immune infiltrates, are associated with improved survival in TNBC. Kaplan-Meier analysis of e OS and f DFS outcomes in women with high versus low densities of CD8⁺PD-1⁺ double positive immune infiltrates. Kaplan-Meier analysis of g OS and h DFS outcomes in women with high versus low CD8⁻PD-1⁺ immune infiltrates