Fig. 3

High PDCD1 and high CD274 mRNA expression is associated with improved survival in TNBC. Kaplan-Meier analysis of a OS and b DFS outcomes in women with high PDCD1 and high CD274 expression, compared with the rest of the cases in the cohort. Both high PDCD1 and high CD274 are associated with improved survival in the METABRIC public TNBC dataset. From the publicly available TNBC dataset, Kaplan-Meier analysis of c OS and d DFS outcomes in women with high PDCD1 and high CD274 compared with the rest of the cases in the cohort (n = 320). High PDCD1 and high CD274 are both associated with increased levels of T cells and MHC-I genes in TNBC, in both public dataset and our NanoString gene expression data. In the group with high PDCD1 and high CD274 expression, a significant increase of certain key DEGs was observed. These genes are associated with T cells and MHC-I molecules, and include CD8A, CD8 CD3D, CD3E, CD3G, HLA-A, HLA-B and HLA-C from e METABRIC and f TCGA, two publically available datasets, and g our NanoString data. Kaplan-Meier analysis of h OS and i DFS outcomes in women with high IFNG expression, compared with low IFNG cases in the cohort. Kaplan-Meier analysis of j OS and k DFS outcomes in women with high IFN associated signature 5 gene expression based on the canonical pathway “Interferon signaling”, compared with the rest of the cases in the cohort