Fig. 2

Vaccination with recombinant MCMV vectors via the IP and SC route induces potent specific T cell responses. Mice were vaccinated with MCMV-IE2-E7 via IP, SC or IN route or kept unvaccinated (control), and the CD8 T cell responses were examined in blood. a Representative flow cytometry plots for MHC class I multimer staining to E7, M38 and M45 epitopes in blood of MCMV-IE2-E7 infected mice via IP during acute phase (day 8 post vaccination) and memory phase (day 64 post vaccination). Numbers represent the percentage of Ag-specific CD8⁺ T cells within the total CD8⁺ T cell population. Control flow cytometry plots show the staining for E7 multimer in unvaccinated mice. Flow cytometry plots show similar numbers of total cells in each plot except for control. b Frequency of Ag-specific CD8⁺ T cells to E7, M38 and M45 epitopes over time. c Frequency of effector-memory (EM) cells (KLRG1⁺, CD62L⁻) within E7, M38 and M45 MHC multimer⁺ CD8⁺ T cells over time. Data represents mean values ± SEM (n = 7–8 mice per group) and are representative of two independent experiments with similar results