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Fig. 6 | Journal for ImmunoTherapy of Cancer

Fig. 6

From: Demarcated thresholds of tumor-specific CD8 T cells elicited by MCMV-based vaccine vectors provide robust correlates of protection

Fig. 6

Pre-existing immunity against the MCMV vector affects the strength of T cell responses and anti-tumor efficacy. a Percentage of OVA, M38 and E7-specific CD8+ T cells, identified using MHC class I multimers. Mice were infected with 1 × 105 PFU via IP or IN MCMV-IE2-OVA at day 0. After 35 days, the mice were vaccinated with 1 × 105 PFU MCMV-IE2-E7 via IP route or with 5 × 105 MCMV-IE2-E7 via the SC route as shown in the schematic. T cell responses were followed for 70 days in blood. Data represent mean values ± SEM. b and c Tumor outgrowth (b) and survival (c) of mice infected with 1 × 105 PFU MCMV-IE2-OVA via IP or IN and vaccinated with 1 × 105 PFU MCMV-IE2-E7 via IP or IN route or with 5 × 105 MCMV-IE2-E7 via SC as shown in the schematic. After 70 days, all the vaccinated and control mice were challenged with 1 × 105 TC-1 tumor cells. The number of tumor-free/total mice is indicated above each tumor outgrowth graph. d The percentage of E7-specific CD8+ T cells within the total CD8+ T cell population in the blood (y-axis) plotted against the E7-specific CD8+ T cell response ranked from low to high (x-axis) of the mice that were either tumor-free (tumor-) or tumor positive (tumor+). Data are representative of two independent experiments with similar results

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