Fig. 2

HMGN1 expanded CD8⁺ T cells in the tumor. a Flow cytometry gating of tumor-infiltrating CD8⁺ T cell populations. b, c The compartment, frequency, and cell number of CD8⁺ T cells in the tumor by using flow cytometry. d, e The compartment, frequency, and cell number of CD44^hi PD-1⁺ CD8⁺ T cells in the tumor. f, g The compartment, frequency, and cell number of CD137⁺ PD-1⁺ CD8⁺ T cells in the tumor. Each result is representative of three independent experiments with at least four mice per group. Data are presented as mean ± SEM. *, P < 0.05, **, P < 0.01 for a dunnett’s post hoc test (compared with control); p values in the figures indicate Student’s t-test comparing mH/αCD4-treated and αCD4-treated groups