Fig. 5

HMGN1/αCD4 treatment synergistically reduced exhausted CD8⁺ T cells in the tumor. a The compartment of PD-1^hi, PD-1^lo, PD-1⁻ CD8⁺ T cells in the tumor. b The expression level of LAG-3, TIM-3, and TIGIT on PD-1^hi CD8⁺ T cells. c The compartment and frequency of IFN-γ⁺ among in PD-1⁺ CD8⁺ T cells. d The compartment and frequency of IL-2⁺ TNF-α⁺ among in IFN-γ⁺ PD-1⁺ CD8⁺ T cells. Each result is representative of three independent experiments with at least four mice per group. Data are presented as mean ± SEM. *, P < 0.05, **, P < 0.01, ***, P < 0.001 for a dunnett’s post hoc test (compared with control); p values in the figure indicate Student’s t-test comparing mH/αCD4-treated and αCD4-treated groups. Geometric mean fluorescence intensity (GMFI)