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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Targeting the tumor stroma with an oncolytic adenovirus secreting a fibroblast activation protein-targeted bispecific T-cell engager

Fig. 1

In vitro characterization of ICO15K-FBiTE. a Schematic structure representation of ICO15K-FBiTE. VL and VH domains of anti-mhFAP and CD3Ɛ are connected by glycine and serine flexible linkers, flanked by the light chain immunoglobulin signal peptide (SP) and the FLAG tag (FT). FBiTE is inserted after the adenovirus fiber gene under the control of the major late promoter (MLP). b Viral production from cell extracts (CE) and supernatants (SN) of ICO15K-FBiTE. A549 cell line was infected with ICO15K or ICO15K-FBiTE. At indicated time points, cell extracts and supernatants were harvested and titrated by an anti-hexon staining-based method. C. Comparative cytotoxicity profile of ICO15K-FBiTE. A549, HT1080 and hCAFs cells were infected with serial dilutions of ICO15K or ICO15K-FBiTE. Cell viability was measured at day 6 post-infection for A549 and HT1080 and at day 7 for hCAFs. Mean values ± SD are plotted in B and C (n = 3). TU, transducing units: number of functional viral particles capable of transducing a cell

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