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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Targeting the tumor stroma with an oncolytic adenovirus secreting a fibroblast activation protein-targeted bispecific T-cell engager

Fig. 2

FBiTE expressed by ICO15K-FBiTE specifically binds to both target and effector cells and is able to activate T cells. a HT1080 (HT), HT-mFAP, HT-hFAP and Jurkat cells were incubated with mock, ICO15K, or ICO15K-FBiTE supernatants and FBiTE binding was detected by flow cytometry. b Average concentration values of IFN-ɣ, TNF-α and IL-2 cytokines were measured by ELISA assay using supernatants from 24 h co-cultures of HEK293 (293), 293mFAP or 293hFAP cells with T cells (E:T = 5) and indicated supernatants. c T-cell proliferation following co-cultures with target cells and indicated supernatants (E:T = 5). A representative result of triplicates is shown. ***, ICO15K-FBiTE significant (P < 0.001) versus mock or ICO15K using one-way ANOVA test with post hoc analysis

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