Skip to main content
Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Targeting the tumor stroma with an oncolytic adenovirus secreting a fibroblast activation protein-targeted bispecific T-cell engager

Fig. 3

Enhanced ICO15K-FBiTE-mediated cytotoxicity of FAP-positive cells. a FBiTE-mediated cytotoxicity was evaluated by flow cytometry after 24 h incubation of CFSE-stained HT1080 cell lines cultured with T cells and indicated supernatants. b CFSE-stained target cells were co-cultured with A549-infected cells and T cells (E:T = 5). After four days of incubation, specific cytotoxicity of CFSE-stained cells was determined by flow cytometry. c Cytotoxicity of CFSE-stained-murine or human CAFs was evaluated. Mean values ± SD are plotted in A, B and C (n = 3). ***, ICO15K-FBiTE significant (P < 0.001) by one-way ANOVA test with post hoc analysis compared to mock and ICO15K

Back to article page