Fig. 6

Depletion of tumor stroma by ICO15K-FBiTE. A549 and HPAC tumors from the antitumor efficacy studies in presence of T cells were harvested at endpoint of the experiments. a-c Piece of tumor samples were mechanically homogenized, total DNA and RNA were extracted and quantification by real-time PCR-based method was performed to evaluate a the virus persistence, b FBiTE expression and c levels of FAP expression in both tumor models. d, e Immunohistochemical stainings of A549 tumors were also performed to assess d presence of virus by staining the early viral protein E1a and e FAP expression by an anti-FAP antibody. *, significant (P < 0.05) by two-tailed unpaired Student’s t-test. ***, significant (P < 0.001) by two-tailed unpaired Student’s t-test