Fig. 7

Cytotoxicity of DNTs is dependent on different mechanisms, which are augmented by IL-15. a Filled histograms represent isotype controls, dotted line histograms and solid line histograms represent DNTs in the absent and presence of IL-15, respectively. Numbers shown are the MFI values. b IFNγ and sTRAIL in the supernatant of DNTs stimulated with or without IL-15 were measured by ELISA. c The percentage of reduced target cell lysis by a specific antibody was compared to those in the presence of the corresponding isotype control antibody. d The percentage of reduced target cell lysis by CMA treatment with or without anti-TRAIL antibody was compared to those in the presence of vehicle DMSO and isotype controls. e NSCLC cell lines were cultured in the presence of media or IL-2/IL-15 conditioned DNT supernatant in the presence of neutralizing anti-TRAIL antibody or isotype control. The death of NSCLC cells was determined by flow cytometry. Statistical differences between different treatment groups were calculated using Two-tailed Student’s t test, for b. One-way ANOVA followed by Bonferroni’s post hoc test in c, d and e. ns, no significant, **P < 0.01, ***P < 0.001. Data from one of two or three representative experiments (b, c, e) or cumulative of six independent experiments (d) are shown