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Table 1 NSCLC cell lines have different susceptibilities towards ex vivo expanded DNTs

From: Human double negative T cells target lung cancer via ligand-dependent mechanisms that can be enhanced by IL-15

Cell line (Cell source) % cytotoxicity (Mean ± SD) at different E:T ratios E:T EC50
20:01 10:01 5:01 2.5:01
H2279 (ATCC) 97.22 ± 12.04 87.69 ± 11.65 47.69 ± 19.34 23.83 ± 13.53 4.03
H460 (ATCC) 89.27 ± 7.23 61.52 ± 8.30 30.09 ± 12.88 14.88 ± 8.75 4.86
12 (PDX) 83.43 ± 17.97 75.32 ± 18.10 50.66 ± 21.09 24.15 ± 10.97 5.76
178 (PDX) 83.03 ± 10.55 58.96 ± 12.30 34.43 ± 7.49 11.81 ± 2.83 6.7
426 (PDX) 83.00 ± 10.50 64.54 ± 13.74 40.79 ± 7.24 16.32 ± 4.46 7.47
277 (PDX) 81.25 ± 9.27 67.62 ± 14.95 47.25 ± 8.07 21.76 ± 6.73 7.56
655 (PDX) 81.14 ± 7.99 63.21 ± 8.85 31.84 ± 1.90 17.79 ± 1.77 7.97
229 (PDX) 80.64 ± 10.41 58.96 ± 10.73 27.98 ± 14.16 10.28 ± 5.43 8.86
H125 (ATCC) 78.62 ± 14.80 53.97 ± 19.28 24.05 ± 10.39 16.39 ± 8.94 9.53
239 (PDX) 63.57 ± 9.98 36.19 ± 16.83 11.82 ± 8.76 12.45 ± 3.81 16.8
A549 (ATCC) 60.12 ± 10.51 35.69 ± 5.41 18.28 ± 5.21 9.77 ± 4.50 16.93
137 (PDX) 53.66 ± 15.40 30.84 ± 13.30 16.67 ± 6.47 8.51 ± 2.90 20.57
  1. Primary and established human NSCLC cell lines were cocultured with ex vivo expanded DNTs at various E:T ratios. The percentages of specific cytotoxicity against target cells were detected. E:T EC50 was calculated for each cell line