Human double negative T cells target lung cancer via ligand-dependent mechanisms that can be enhanced by IL-15

Table 1 NSCLC cell lines have different susceptibilities towards ex vivo expanded DNTs

Cell line (Cell source)	% cytotoxicity (Mean ± SD) at different E:T ratios				E:T EC50
Cell line (Cell source)	20:01	10:01	5:01	2.5:01	E:T EC50
H2279 (ATCC)	97.22 ± 12.04	87.69 ± 11.65	47.69 ± 19.34	23.83 ± 13.53	4.03
H460 (ATCC)	89.27 ± 7.23	61.52 ± 8.30	30.09 ± 12.88	14.88 ± 8.75	4.86
12 (PDX)	83.43 ± 17.97	75.32 ± 18.10	50.66 ± 21.09	24.15 ± 10.97	5.76
178 (PDX)	83.03 ± 10.55	58.96 ± 12.30	34.43 ± 7.49	11.81 ± 2.83	6.7
426 (PDX)	83.00 ± 10.50	64.54 ± 13.74	40.79 ± 7.24	16.32 ± 4.46	7.47
277 (PDX)	81.25 ± 9.27	67.62 ± 14.95	47.25 ± 8.07	21.76 ± 6.73	7.56
655 (PDX)	81.14 ± 7.99	63.21 ± 8.85	31.84 ± 1.90	17.79 ± 1.77	7.97
229 (PDX)	80.64 ± 10.41	58.96 ± 10.73	27.98 ± 14.16	10.28 ± 5.43	8.86
H125 (ATCC)	78.62 ± 14.80	53.97 ± 19.28	24.05 ± 10.39	16.39 ± 8.94	9.53
239 (PDX)	63.57 ± 9.98	36.19 ± 16.83	11.82 ± 8.76	12.45 ± 3.81	16.8
A549 (ATCC)	60.12 ± 10.51	35.69 ± 5.41	18.28 ± 5.21	9.77 ± 4.50	16.93
137 (PDX)	53.66 ± 15.40	30.84 ± 13.30	16.67 ± 6.47	8.51 ± 2.90	20.57

Primary and established human NSCLC cell lines were cocultured with ex vivo expanded DNTs at various E:T ratios. The percentages of specific cytotoxicity against target cells were detected. E:T EC50 was calculated for each cell line