Fig. 4
From: HDAC6 selective inhibition of melanoma patient T-cells augments anti-tumor characteristics
ACY-1215 increases the percentage of central memory T-cells. T-cells isolated from melanoma patient PBMC samples were expanded with DMSO or ACY-1215 500nM. (a) Paired analysis of CD45RA-CD45RO + CD62L + CCR7+ percentages after DMSO versus ACY-1215 treatment of five patient samples assessed. (b) CD8+ and CD4+ T-cells from six patients (P01–06) were assessed by flow cytometry for relative changes in the subsets: CD45RA-CD62L+ (central memory, Tcm), CD45RA + CD62L+ (Tnaïve), CD45RA-CD62L- (effector memory, Tem) and CD45RA + CD62L- (effector, Teff). Changes were calculated relative to DMSO, illustrated by the vertical black lines. (a-b) Samples were evaluated across three independent experiments. **p < 0.01