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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Characterization of immune responses to anti-PD-1 mono and combination immunotherapy in hematopoietic humanized mice implanted with tumor xenografts

Fig. 1

Anti-PD-1 therapy inhibits growth of TNBC xenograft in hu-CB-BRGS model. a, PBMCs of humanized mice were analyzed pre-tumor engraftment for expression of mouse (mCD45) and human (hCD45) hematopoietic markers (left panels). The hCD45+ cells were analyzed for expression of T-cell (CD3), B-cell (CD20), and CD8+ T-cell subsets (middle, right panels). A mixture of CB PBMCs and mouse spleen cells served as a positive staining control (top panels). b, Expression of PD-1 on T-cells (hCD45+ CD3+) in PBMCs from a control CB (top) and a 16-week old humanized mouse (bottom). The MFI of PD-1 on CD4+ (black) and most CD8+ (red) T-cells increase with age (right, CD4: P = 0.04; CD8: ns). Chimerism and PD-1 staining results represent > 100 hu-CB-BRGS mice. c, Growth curves of TNBC MDA-MB-231 tumors in nivolumab-treated (blue line) or untreated (black line) hu-CB-BRGS mice (left, n = 6 tumors/3 mice per group) and non-humanized BRGS mice (right, n = 2 tumors per group). P-values: * < 0.05

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