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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Characterization of immune responses to anti-PD-1 mono and combination immunotherapy in hematopoietic humanized mice implanted with tumor xenografts

Fig. 4

Few B-cells and many myeloid human cells in TILs of TNBC-engrafted hu-CB-BRGS mice. a, No change observed in human B-cell frequencies (left, middle panels) or function (hIgM and IgG concentrations in serum, right panel) in tumor-bearing, humanized mice with nivolumab treatment. b, Decreased human myeloid cell frequencies in TNBC tumors treated with nivolumab. The upper plots depict the flow cytometry gating strategy for identification of the CD4 + CD3- myeloid population among hCD45+ cells from hPBMC (staining control), and indicated organs of a humanized mouse engrafted with TNBC cell line and data from each mouse is shown in upper right graphs. The lower plots show flow cytometry gating (left) for myeloid populations. The myeloid cells are also identified as hCD45+, CD3-, CD19-, (CD14+, CD33+, CD11b + or CD11c+) and the frequency of the HLA-DRlo/− population among these cells is shown. Each dot represents data from the indicated organ from an individual humanized mouse that was either untreated (−, black) or treated with nivolumab (+, blue) for 11 or 21 days, as indicated. P-values: * < 0.05

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