Fig. 5From: The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancerASC is expressed in situ in tumor cells and TAMs and its expression in primary tumors correlates with reduced overall survival. a-d Immunohistochemistry of ASC protein expression in representative samples of tumor and tumor surrounding tissue. a ASC staining in both the nucleus and the cytoplasm in both tumor cells and TAMs. b ASC staining in both the nucleus and the cytoplasm in tumor cells while prevalently cytoplasmic in TAMs. c ASC staining is negative in tumor cells and in TAMs is prevalently nuclear. d ASC staining in normal pancreatic tissue surrounding the tumor. e Immunofluorescence staining of ASC (green) in tumor cells and TAMs (identified by CD163 staining in red). Cells co-expressing ASC and CD163 are indicated in orange (merge). f Kaplan-Meier curves of overall survival of 178 PDAC patients grouped based on median ASC RNA expression. Significance was evaluated by the Log-rank (Mantel-Cox) test (p = 0.04)Back to article page