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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Efficacy of PD-1 blockade in cervical cancer is related to a CD8+FoxP3+CD25+ T-cell subset with operational effector functions despite high immune checkpoint levels

Fig. 4

Phenotyping and functional status of CD8+FoxP3+CD25+ T cells vs. CD8+FoxP3 T cells. a t-SNE density plots showing expression distribution within the CD8+ T cell population of FoxP3, CD25, PD-1, TIM-3, LAG-3, and CTLA-4; representative results from matched LN- and LN+ samples from the same patient (a) are shown and from another SLN+ sample (b). NB: both LN+ samples showed positive response to HPV16 E6, which was enhanced by PD-1 blockade (no response in LN-). Box indicates the CD8+FoxP3+CD25+ population. b Immune checkpoint receptor profiling of CD8+FoxP3+CD25+ T cells versus CD8+FoxP3 T cells (LN+, n = 13). c Frequency of the effector and memory markers CD127, CD27 and CD45RA on the CD8+FoxP3 vs. CD8+FoxP3+CD25+ subsets in LN+ (n = 12). d PD-1 expression on CD8+FoxP3, CD8+FoxP3CD25+, and CD8+FoxP3+CD25+ T cells in LN+ (n = 13). e Frequency of CD8+FoxP3+ vs. CD8+FoxP3 T cells (co-)expressing intracellular Granzyme-B (GrB), IFNγ, IL-2 and TNFα upon o/n anti-CD3 stimulation in LN+ (n = 4) and PT (n = 3). Error bars represent standard error of the mean. *P = 0.01 to 0.05, **P = 0.001 to 0.01, ***P = 0.001 to 0.0001

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