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Fig. 5 | Journal for ImmunoTherapy of Cancer

Fig. 5

From: Efficacy of PD-1 blockade in cervical cancer is related to a CD8+FoxP3+CD25+ T-cell subset with operational effector functions despite high immune checkpoint levels

Fig. 5

Phenotype, distribution, and localization of CD8+FoxP3+ T cells in cervical tumor tissue. a Representative immunofluorescence staining showing FoxP3 (in orange), CD8 (in blue), CD3 (in red), PD-1 (in yellow), GrB (in green), and the merged image including DAPI (in grey). Boxes denote CD8+FoxP3+ T cells. Scale bar in merged image is 50 μm. In merged image, T (tumor) represents intratumoral area and S (stroma) represents peritumoral area. Scatter plot showing peritumoral and intratumoral rates for b CD8+FoxP3+ T cells, and percentages of c PD-1-expressing CD8+FoxP3+ T cells and d GrB-expressing CD8+FoxP3+PD-1+ T cells in tumor-positive lymph nodes (n = 4 for LN+, ●) and primary tumors (n = 4 for PT, ▲). Error bars represent standard error of the mean. *P = 0.01 to 0.05

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