Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Increased intratumoral mast cells foster immune suppression and gastric cancer progression through TNF-α-PD-L1 pathway

Fig. 4

Tumor-derived TNF-α activates NF-κB pathway to induce PD-L1 expression on mast cells. a Expression of PD-L1 on hCBMCs exposed to TTCS or TNF-α with or without BAY 11–7082 (an IκBα inhibitor) for 24 h. black, isotype control. b PD-L1, p65 and p-p65 on/in LAD2 cells exposed to autologous TTCS, NTCS, or TTCS with anti-TNF-α antibody for 24 h (detecting for PD-L1) or 3 h (detecting for p65 and p-p65) were analyzed by western blot. c PD-L1 on HMC-1 cells exposed to TTCS with or without BAY 11–7082 (an IκBα inhibitor) for 24 h was analyzed by immunofluorescence staining. Green, PD-L1; blue, DAPI-stained nuclei. Scale bars: 20 μm. d PD-L1, p65 and p-p65 on/in LAD2 cells exposed to TTCS with or without BAY 11–7082 (an IκBα inhibitor) were analyzed by western blot

Back to article page