Fig. 2

NKG2D blockade during effector phase led to the generation of defective cytokine responses by memory cells. Memory pMel CD8 T cells were generated as in Fig. 1a. a Representative graph shows the percentage of memory pMel cells (CD90.1⁺) among total CD8 T cells present in the spleen one day after the in vivo killing assay. b-g Splenocytes from (A) were restimulated overnight with hgp100 peptide or gp33 (irrelevant peptide). Cytokine production by CD90.1⁺ pMel cells was measured the next day by flow cytometry. The percentage of memory pMel CD8 T cells that produce 0 (b) or 3 cytokines (c) is shown. d-f Shown are flow examples and graphs summarizing the percentage of pMel CD8 T cells secreting IFN-γ (d), TNF-α (e) or IL-2 (f). g Pie charts show the percentage of pMel cells producing 1, 2, or 3 cytokines, among the cells that produce at least one cytokine (denoted above each pie chart). For (b-g), 2 mice/group were excluded due to too low frequency of recovered pMel cells. Data shown are representative of three independent experiments