Fig. 3

Blocking NKG2D during effector responses of endogenous CD8 T cells resulted in highly defective memory responses (a) Example of the in vivo killing assay readout by flow cytometry during memory responses. b Graph shows the percentage of antigen-experienced (CD44⁺) CD8 T cells among total CD8 population present in the spleen one day after the in vivo killing assay. c-h Splenocytes from (b) were restimulated overnight with gp33 peptide or irrelevant peptide (hgp100 peptide). Cytokine production was measured the next day by flow cytometry. The percentages of endogenous CD44⁺ CD8 T cells that produce 0 (c) or 3 cytokines (d) are shown. e-g Shown are flow examples and graphs summarizing the percentage of endogenous CD44⁺ CD8 T cells secreting IFN-γ (e), TNF-α (f) or IL-2 (g). h Pie charts show the percentage of endogenous CD44⁺ CD8 T cells secreting 1, 2, or 3 cytokines among the cells that produce at least one cytokine (denoted above each pie chart). Data shown are representative of two independent experiments