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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: NKG2D signaling certifies effector CD8 T cells for memory formation

Fig. 4

NKG2D signaling is not needed on activated pMel CD8 T cells for proper effector functions. Effector pMel CD8 T cells were generated as described in Fig. 1a. One day before the in vivo killing assay, half of the mice were injected with the anti-NKG2D blocking antibody. a Example of the in vivo killing assay readout by flow cytometry during memory responses. b Graph shows the percentage of effector pMel CD8 T cells (CD90.1+) among total CD8 T cells present in the spleen one day after the in vivo killing assay. c-h Splenocytes from (b) were restimulated overnight with hgp100 peptide or gp33 peptide. Cytokine production was measured the next day by flow cytometry. The percentages of effector pMel CD8 T cells (CD90.1+) that produce 0 (c) or 3 cytokines (d) are shown. e-g Shown are flow examples and graphs summarizing the percentage of pMel CD8 T cells secreting IFN-γ (e), TNF-α (f) or IL-2 (g). h Pie charts show the percentage of pMel cells producing 1, 2, or 3 cytokines, among the cells that produce at least one cytokine (denoted above each pie chart). Data shown are representative of five independent experiments

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