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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: The immune suppressive microenvironment of human gliomas depends on the accumulation of bone marrow-derived macrophages in the center of the lesion

Fig. 2

MG and BMDM characterization in different glioma areas and analysis of their immune suppressive activity. a Surgical microscopic view under blue light (left panel) and preoperative Magnetic Resonance T1-weighted image after gadolinium administration (right panel) of a patient with a left deep GBM. Different fluorescence intensities are detected in distinct tumor areas: a bright fluorescence corresponds to the ring tumor enhancement at MRI, a dim fluorescence is present in the peritumoral infiltration, lack of fluorescence is in the central necrotic area. b Representative flow cytometry panels and (c), cumulative data of BMDM (green) and MG (blue) cells in three tumor layers identified by 5-ALA fluorescence in GBM tissues (n = 24 core, n = 30 intense fluorescence, n = 19 marginal samples) (left histograms) and from grade II (n = 11) and III (n = 9) glioma patients (right plots). d Immunosuppressive activity of BMDM (green) and of MG (blue) isolated by FACS sorting from the central intense fluorescence layer, or from the surrounding peritumoral space of GBM patients (n = 7 for BMDM in the center, n = 4 for MG in the center; n = 3 for BMDM in the margin, 4 for MG in the margin). MG tested from grade II (n = 2) and grade III (n = 3) gliomas. Comparison by Mann-Whitney test, ***p < 0.001

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