Fig. 7
Non-invasive visualization of transient splenomegaly caused by massive, transient expansion of CD122+ lymphocytes. a Masses and photographs of representative spleens from untreated mice, mice after a 5-day-long treatment with IL-2c, and 7 days after the last IL-2c injection. b Micro-CT images of mice treated with IL-2c for 5 days or not. ExiTron TM 12000 nanoparticles were injected for enhanced spleen contrast. c ImmunoPET with 64Cu-NOTA-anti-PD-L1. Untreated mice and mice treated for 5 days with IL-2c received 20 μg 64Cu-NOTA-anti-PD-L1 (~ 6 MBq); PET images were acquired 24 h later. Representative coronal and transverse PD-L1 immunoPET/CT sections are shown. White ticks in the C-sections indicate the positions of the T- and S-sections. d Total cell counts of spleen and lymph nodes. e Strong transient expansion of CD122+ CD8+ T cells after treatment with IL-2c as assessed by flow cytometry