Fig. 2
Relationship between T cell dynamics and oxygen distribution in bone marrow of mice with or without B-ALL. a Low magnification confocal images and high magnification FaST-PLIM images of the calvaria. FaST-PLIM images are for the fields of view indicated with the dashed squares. “b” indicates the areas of bone and no signal from the probe. The specimens are exposed skulls in anesthetized mice transgenic for CD2-DsRed (red, T cells) / CD11c-YFP (green, myeloid cells) and infused with blood tracer dye dextran-TRITC and PtP-C343 probe. The blue represents B-ALL-mCerulean fluorescence in the mouse inoculated, about 2 weeks earlier, with the leukemia. b Mean oxygen tension in the entire BM cavity of non-leukemic (“NL”), end-stage leukemic (“ESL”), and asphyxiated (“D”) mice. Each symbol represents a single FOV. c Oxygen experience of bone marrow T cells. Each point represents a readout for one T cell in one time point (n > 30,000 per group). d Overall relation between the average T cell velocity and pO2. The range was analyzed in intervals of 5 mmHg. e Comparison of T cell velocities in pO2 below or above 5 mmHg in leukemic mice. Each symbol represents a T cell instance (n > 30,000 per group). Pooled from more than 2 FOVs per mouse in at least 3 mice per group. Symbol * denotes p < 0.05 determined by Student’s T-test. Error bars show standard deviations