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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Merger of dynamic two-photon and phosphorescence lifetime microscopy reveals dependence of lymphocyte motility on oxygen in solid and hematological tumors

Fig. 3

Oxygen distribution and T cell dynamics in solid MCA tumors in lungs, and T cell response to supplementary oxygen. a Intravital FaST-PLIM images of solid tumor in the lung of a mouse transgenic for hCD2-DsRed (T cell reporter, red) and CD11c-YFP (dendritic cell/macrophage reporter, green). Cancer cells (MCA-205) express mCerulean fluorescence (blue). Blood supply was labeled with TRITC-dextran (purple), co-infused with PtP-C343. T cells were tracked for 30 min and shown is one time point. The lifetime is that of PtP-C343 phosphorescence, contemporaneous with the corresponding fluorescence images. b pO2 experienced by T cells in the tumor margin (TM) vs. tumor core (TC) during the mouse breathing air. c T cell instantaneous velocities in the local pO2 < 5 mmHg vs. that in pO2 > 5 mmHg. Each symbol represents a T cell instance (n > 5000). Graphs display data from representative tumor FOVs. * denotes p < 0.05 determined by Student’s T-test. Vertical bars represent standard deviations

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