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Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Immune-enrichment of non-small cell lung cancer baseline biopsies for multiplex profiling define prognostic immune checkpoint combinations for patient stratification

Fig. 2

Highly proliferating, effector TIL and TIL-B densities are linked to positive prognostic of NSCLC patients. a Summarizing graph of P-values generated from K-M survival analyses of markers applied to IIC-enriched biopsy TMA, where significance indicates positive associations of IIC subsets, and proliferation and effector molecules with OS. b K-M curves (top) from Ki-67 co-labeling with CD45+ IICs or CD3+ TILs on TMA, and representative close-up IF images from cores (bottom) demonstrating co-labeling on cells. c K-M curves (top) from CD4+ and CD8+ TILs on TMA, with representative close-up IF images from cores (bottom) demonstrating their co-labeling CD3+ TILs. d K-M curves (top) from CD20+ TIL-Bs, PNAd+ HEV, and CD68+ TAMs, with representative close-up IF images from cores (bottom). e Graph of average proportions of IIC subsets relative cell count (DAPI), where percentages represent IIC subset abundance relative to CD45+ IICs. Percentages are relative to CD45 content, and error bars represent mean ± s.d.. f Graph of correlations between IIC subsets and quantified effector molecules (IFN-γ, GZMB, HLA-DR). Percentages represent IIC subset attribution to effector molecule expression, as calculated from proportions of individual IIC subsets infiltrating cores expressing effector molecules. g K-M curves (top) of GZMB, IFN-γ, and HLA-DR effector markers, with representative close-up IF images from cores (bottom) of these markers and TILs. The number of patients (n) for each group is given on K-M curves, and remainder are in Additional file 1: Figure S2b. Algorithm design, normalization and analyses were performed by two independent operators. Norm., normalized; hi, high marker expression, lo, low marker expression; μm, micron; P, Log-rank test; ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; HR, hazard ratio (Log-rank); CI, confidence interval of ratio; NA, not applicable

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