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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Immune-enrichment of non-small cell lung cancer baseline biopsies for multiplex profiling define prognostic immune checkpoint combinations for patient stratification

Fig. 3

Effects of ICP expression on NSCLC patients. a Summarizing graph of P-values generated from K-M survival analyses of IIC-enriched TMA, where significance indicates positive associations ICP and CD3-ICP co-labeling cells with OS (top left). K-M curves and representative close-up IF images from cores (right and bottom) of CD3 dense core areas, demonstrating colocalization between CD3 and TIGIT, TIM-3, LAG-3, BTLA, or PD-1. b Summarizing graph of P-values generated from K-M survival analyses of IIC-enriched TMA, where significance indicates positive associations of combinations of CD3, CD8, PD-1, PD-L1 and TIM-3 with OS (top). Representative close-up IF images from cores (bottom) of CD3 dense core areas, demonstrating colocalization between these antigens. The number of patients (n) for each group is given on K-M curves, and remainders are (high and low, respectively): CD3 n = 34hi, 32lo; CD8 n = 41hi, 21lo; TIGIT n = 26hi, 38lo, TIM-3 n = 21hi, 26lo, LAG-3 n = 29hi, 33lo, BTLA n = 30hi, 30lo, PD-1 n = 36hi, 30lo, CD3-PD-1 n = 29hi, 24lo; CD8-PD-1 n = 36hi, 32lo; TIM-3-PD-1 n = 34hi, 30lo; PD-L1 n = 25hi, 33lo; CD3-PD-L1 n = 18hi, 30lo; CD8-PD-L1 n = 19hi, 24lo; TIM-3-PD-L1 n = 25hi, 34lo. Algorithm design, normalization and analyses were performed by two independent operators. hi, high marker expression, lo, low marker expression; μm, micron; merge, merge of all IF channels; P, Log-rank test; ns, not significant (implied when no asterisk is present); * P < 0.05; ** P < 0.01; HR, hazard ratio (Log-rank), CI, confidence interval of ratio

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