Fig. 3
From: Antisense oligonucleotide targeting CD39 improves anti-tumor T cell immunity
Improved human CD8+ T cell proliferation in the presence of hCD39-specific ASO and extracellular ATP. Human CD8+ T cells were labelled with cell proliferation dye, activated with anti-CD3, anti-CD28 and IL-2, and treated with 5 μM of the antisense oligonucleotide A04040H or the control oligo 2 for a total treatment time of five days. Subsequently, 400 μM of ATP was added to cells on day three and day four after start of oligonucleotide-treatment. On day five after start of oligonucleotide treatment, (a) hCD39 protein expression, (b) proliferation index, (c) relative cell numbers of CD8+ T cells relative to mock-treated cells without ATP (set as 1) as wells as (d) % living cells were analyzed using flow cytometry. Cells were derived from two different donors, processed on three different dates. Bar graphs depict the mean of three independent experiments run in triplicates +/− SD. Asterisks indicate significant differences between the respective conditions