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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Interleukin-1 alpha increases anti-tumor efficacy of cetuximab in head and neck squamous cell carcinoma

Fig. 1

Cetuximab induces secretion of pro-inflammatory cytokines via IL-1R/MyD88 signaling. Cal 27 and SQ20B HNSCC cells (a-c), SQ20B cells derived from MyD88 stable knockout clones (shMyD88 #2, shMyD88 #9) and control cells (shControl) (d-f), and SQ20B cells derived from IL-1R1 stable knockout clones (shIL-1R #1, shIL-1R #2) and control cells (shControl) (g-i) were treated with 1–100 μg/mL cetuximab (CTX) or 100 μg/mL IgG for 48 H. media was collected and ELISAs were performed to measure IL-1α (a, d, g), IL-6 (b,e,h), and IL-8 (c,f,i). Cells were analyzed for expression of MyD88 (D inset) and IL-1R1 (G inset) by Western blot and β-actin was used as a control. Error bars = SEM. N = 3. ¥:p < 0.05 vs. respective IgG treatment; *p < 0.05 vs. respective shControl cell line. ND = not detected

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