Fig. 6

The anti-tumor effects of cetuximab+IL-1α-NP are T cell dependent. Female BALB/c mice (n = 9–10 mice/treatment group) bearing TUBO-EGFR tumors were treated with cetuximab (CTX, 8 mg/kg twice/week) in combination with a single i.p. dose on treatment day 1 of IL-1α-NPs (0.5 mg NPs containing 7.5 μg IL-1α) (CTX + IL-1α-NP (a,b) with or without anti-CD4 (100 μg (clone GK1.5)) (a,c) or anti-CD8 (300 μg (clone 53–6.7)) (a,d) 1 and 3 days prior to tumor inoculation, and every 3–4 days after tumor inoculation. Treatment duration was 3 weeks. Tumor volumes were measured three times per week. Tumor growth curves shown were stopped after a mouse in any treatment group reached euthanasia criteria. Error bars = SEM. *:p < 0.05. b-d: Shown are spaghetti plots for each individual mouse in each treatment group shown in a. Tumors from female BALB/c mice bearing TUBO-EGFR tumors (n = 3–4) were treated as described in A and harvested after 2 weeks of therapy for validation of CD4⁺ T cell (E) and CD8⁺ T cell (F) depletion by flow cytometry. *:p < 0.05 vs NT, **:p < 0.05 vs anti-CD4. Error bars = SDM