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Fig. 1 | Journal for ImmunoTherapy of Cancer

Fig. 1

From: Cocultures of human colorectal tumor spheroids with immune cells reveal the therapeutic potential of MICA/B and NKG2A targeting for cancer treatment

Fig. 1

Allogeneic activated/memory T and NK cells are able to infiltrate HT29 tumor spheroids. a Scheme of the coculture (CC) protocol between HT29 spheroids and CD19-CD14- sorted PBMCs from healthy donors. b Immunofluorescence (n = 2 independent experiments) and flow cytometry (n = 19 independent experiments) analyses of spheroid immune infiltration in the presence or not of IL-15 at 24 h. c Flow cytometry analyses of T and NK cells (respectively gated CD3+ and CD3-CD56+ among live single cells lymphocytes) as well as CD4+ and CD8+ T cells subsets (respectively gated CD4 + CD8- and CD4-CD8+ among CD3+) percentages in the IN and OUT compartments, in the presence or not of IL-15 at 24 h. n = 19 independent experiments. d Flow cytometry analyses of CD25, CD107a and CD45RO expression by CD4+ T cells, CD8+ T cells and NK cells in the IN and OUT compartments in the presence or not of IL-15 at 24 h. n = 8 to 18 independent experiments. Statistical significance of immunofluorescence experiments was analyzed using the Mann-Whitney test, the others using Wilcoxon matched-pairs signed rank test (* p < 0.05; ** p < 0.005, *** p < 0.001, **** p < 0.0001)

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