Skip to main content
Fig. 2 | Journal for ImmunoTherapy of Cancer

Fig. 2

From: Cocultures of human colorectal tumor spheroids with immune cells reveal the therapeutic potential of MICA/B and NKG2A targeting for cancer treatment

Fig. 2

Immune infiltration and activation in HT29 spheroids lead to tumor cell apoptosis and spheroid destruction. HT29 spheroids were cocultured or not with CD19-CD14- PBMCs and IL-15 and we monitored a spheroid volume using microscopic pictures, b the dynamic cleavage of caspase-3/− 7 in the spheroids using Incucyte live imaging system, and c the staining by Annexin V and DAPI dyes of EpCAM+ cells by flow cytometry at 48 h. n = 4 to 12 independent experiments. Statistical significance of A panel was analyzed using 2-way ANOVA, the other panels using the Wilcoxon matched-pairs signed rank test (* p < 0.05; ** p < 0.005, *** p < 0.001, **** p < 0.0001)

Back to article page