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Fig. 3 | Journal for ImmunoTherapy of Cancer

Fig. 3

From: Cocultures of human colorectal tumor spheroids with immune cells reveal the therapeutic potential of MICA/B and NKG2A targeting for cancer treatment

Fig. 3

Specific effects of CD4, CD8 and NK cells cocultured with HT29 spheroids alone or combined. HT29 spheroids were cocultured with sorted CD4 T cells, CD8 T cells and NK cells at a 1:1 ratio, alone or in combination and with or without IL-15. We analyzed a spheroid destruction through analysis of macroscopic pictures and b tumor cell apoptosis using flow cytometry after 48 h as well as c immune infiltration and d expression of CD25, CD107a and CD45RO by CD4 T cells, CD8 T cells and NK cells in IN and OUT compartments after 24 h. n = 4 to 5 independent experiments. Statistical significance was analyzed using the Wilcoxon matched-pairs signed rank test (# p = 0.06, as compared to spheroid alone in A to C panels and to OUT cells in D panels)

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