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Fig. 4 | Journal for ImmunoTherapy of Cancer

Fig. 4

From: Cocultures of human colorectal tumor spheroids with immune cells reveal the therapeutic potential of MICA/B and NKG2A targeting for cancer treatment

Fig. 4

NKG2D-MICA/B pathway is engaged during the cocultures. a NKG2D expression by CD4 T cells, CD8 T cells and NK cells in the IN and OUT compartments, in the presence or not of IL-15, as measured by flow cytometry at 24 h. n = 18 independent experiments b MICA/B expression by tumor cells in the spheroids cocultured or not with CD19-CD14- PBMCs, as measured by immunohistochemistry at 24 h. Representative pictures of 1 experiment. c to e Analyses of c spheroid volume, d tumor cell apoptosis and e spheroid infiltration 48 h after coculturing HT29 spheroids with CD19-CD14- PBMCs in the presence or not of anti-NKG2D blocking antibodies. n = 3 to 4 independent experiments. Statistical significance of A panel was analyzed using the Wilcoxon matched-pairs signed rank test, C to E panels using paired t test (* p < 0.05; ** p < 0.005, *** p < 0.001, **** p < 0.0001)

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